InhaltsangabePART I: Expression and Activity of Plant MAPKs1. Transient Expression in Arabidopsis Leaf Mesophyll Protoplast System for Cell-based Functional Analysis of MAPK Cascades SignalingJong Hee Im and Sang-Dong Yoo 2. Quantification of Stress Induced Mitogen-activated Protein Kinase Expressional Dynamic using Quantitative Real-time PCRPavel Krenek and Veronika Smékalová 3. Analysis of MAPK Activities using MAPK-specific AntibodiesRoland Willmann, Daniel J. Haischer, and Andrea A. Gust 4. Detection of Protein Phosphorylation and Charge Isoforms using Vertical one-Dimensional Isoelectric Focusing GelsJeffrey C. Anderson and Scott C. Peck 5. Affinity-based SDS PAGE Identification of Phosphorylated Arabidopsis MAPKs and Substrates by Acrylamide Pendant Phos-TagGeorge Komis, Tomás Takác, Slávka Bekesová, Pavol Vadovic, and Jozef Samaj PART II: Genetic Manipulation of MAPK Function 6. Identification of Constitutively Active AtMPK6 Mutants using a Functional Screen in Saccharomyces CerevisiaeElodie Hudik, Souha Berriri, Heribert Hirt, and Jean Colcombet 7. Virus-induced Gene Silencing in Plant MAPK ResearchChristian Hettenhausen, Ian T. Baldwin, and Jianqiang Wu 8. RNA Interference of Plant MAPK Cascades for Functional StudiesJuan Xu and Shuqun Zhang PART III: Localization Studies of MAPKs and Their Interactions9. Immunofluorescent Localization of MAPKs and Colocalization with Microtubules in Arabidopsis Seedling Whole Mount ProbesOlga Samajová, George Komis, and Jozef Samaj 10. Immunofluorescent Localization of MAPKs in Steedman's Wax SectionsMiroslav Ovecka, Olga Samajová, Frantisek Baluska, and Jozef Samaj 11. Fluorescent Protein Tagging of Arabidopsis MAPKs for In Vivo Localization StudiesAnna Doskocilová, Ivan Luptovciak, Veronika Smékalová, and Jozef Samaj 12. Bimolecular Fluorescent Complementation (BiFC) by MAP Kinases and MAPK PhosphatasesAlois Schweighofer, Volodymyr Shubchynskyy, Vaiva Kazanaviciute, Armin Djamei, and Irute Meskiene Part IV: Analysis of MAPK Substrates 13. Determination of Phosphorylation Sites in Microtubule Associated Protein MAP65-1Andrei Smertenko 14. In vivo Phosphorylation of WRKY Transcription Factor by MAPKNobuaki Ishihama, Hiroaki Adachi, Miki Yoshioka,  and Hirofumi Yoshioka 15. Rapid Mutagenesis-based Analysis of Phosphorylation Sites in Mitogen-activated Protein Kinase SubstratesLennart Eschen-Lippold, Nicole Bauer, Julia Löhr, Mieder A.T. Palm-Forster, and Justin Lee PART V: Large Scale Studies on MAPKs, Substrates, and Interactions 16. Yeast Two-hybrid System for Dissecting the Rice MAPK InteractomeRaksha Singh, Sarmina Dangol, and Nam-Soo Jwa 17. Experimental and Analytical Approaches to Characterize Plant Kinases using Protein MicroarraysElizabeth K. Brauer, Sorina C. Popescu, and George V. Popescu  18 Protein Complexes Characterization in Arabidopsis thaliana by Tandem Affinity Purification Coupled to Mass Spectrometry AnalysisJean Bigeard, Delphine Pflieger, Jean Colcombet, Loïc Gérard, Hakim Mireau and Heribert Hirt 19 Quantitative Phosphoproteomic Analysis using iTRAQ MethodAsano T and Nishiuchi T

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