This volume provides an overview of modern and emerging methods for production, analysis, and utility of peptide libraries. Chapter focus on methods and techniques for synthesis, genetic expression, hybrid synthesis-expression, examples of modern utility of these libraries, de novo discovery of reactions, hybrid organic-inorganic materials and, emerging tools for the analysis of these libraries by method of genetic selection and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Peptide Libraries: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Hersteller:
Humana Press in Springer Science + Business Media
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InhaltsangabeI. Chemical Strategies for Library Synthesis 1. Design, Synthesis and Application of OB2C Combinatorial Peptide and Peptidomimetic LibrariesKit Lam and Ruiwu Liu  2. Synthesis of Macrocyclic Organo-Peptide Hybrids from Ribosomal Polypeptide Precursors via CuAAC-/Hydrazide-Mediated CyclizationJessica M. Smith and Rudi Fasan 3. Synthesis and Screening of One-Bead-One-Compound Cyclic Peptide LibrariesZiqing Qian, Punit Upadhyaya, and Dehua Pei 4. Creating Site-Specific Isopeptide Linkages Between Proteins with the Traceless Staudinger LigationKristen A. Andersen and Ronald T. Raines 5. Macrocyclic Templates for Library Synthesis of Peptido-Conjugates Serge Zaretsky, Joanne Tan, Jennifer L. Hickey, and Andrei K. Yudin 6. Synthesis and Alkylation of aza-Gly-Pro Building Blocks of Peptidomimetic Libraries for Developing Prostaglandin F2a Receptor Modulators as Therapeutics to Inhibit Preterm LabourCarine B. Bourguet and William D. Lubell II. Chemistry and Genetically-Encoded Peptide Libraries 7. A Fragment Based Selection Approach for the Discovery of Peptide Macrocycles Targeting Protein KinasesElizabeth Restituyo, Karla Camacho-Soto, and Indraneel Ghosh 8. Peptide and Peptide Library Cyclization Via Bromomethyl Benzene DerivativesDavid E. Hacker, Mohammed Almohaini, Aruna Anbazhagan, Zhong Ma, and Matthew C. T. Hartman 9. Phage Selection of Bicyclic Peptides Based on Two Disulfide BridgesShiyu Chen and Christian Heinis  10. De Novo Discovery of Bioactive Cyclic Peptides Using Bacterial Display and Flow CytometryAmol V. Shivange and Patrick S. Daugherty 11. Chemical Post-Translational Modification of Phage-Displayed PeptidesSimon Ng, Katrina F. Tjhung, Beth M. Paschal, Christopher J. Noren, and Ratmir Derda 12. Mapping Protein-Protein Interactions with Phage-Displayed Combinatorial Peptide Libraries and Alanine-ScanningMalgorzata E. Kokoszka and Brian K. Kay 13. Identifying Reactive Peptides from Phage-Displayed LibrariesGlenn M. Eldridge and Gregory A. Weiss 14. The Best and the Brightest: Exploiting Tryptophan-Sensitized Tb3+ Luminescence to Engineer Lanthanide-Binding TagsLangdon J. Martin and Barbara Imperiali III. Utility of Peptide Libraries 15. Synthesis and Cell-Based Screening of One-Bead One-Compound Peptide Libraries        F.C. Bononi and Len G. Luyt 16. Screening Peptide Array Library for the Identification of Cancer Cell Binding PeptidesKamaljit Kaur, Sahar Ahmed, Rania Soudy, and Sarfuddin Azmi 17. Next Generation Sequencing of Phage-Displayed Peptide Libraries Ratmir Derda and Wadim Matochko 18. Maleimide-Based Method for Elaboration of Cysteine-Containing Peptide Phage Libraries Buyung Santoso and Brion W. Murray