Now fully updated and considerably expanded, Glycoanalysis Protocols, 2nd ed., makes available to all protein scientists, and particularly those working with today's pharmaceuticals, the most advanced and reproducible glycoanalysis techniques currently in use. Developed by highly experienced carbohydrate chemists, biochemists, and physical chemists, these detailed, up-to-date, and proven analytical techniques cover the areas of glycoprotein macromolecular structural analysis, oligosaccharide profiling, lipid conjugate characterization, microorganism structure determination, and proteoglycan function. Special attention has been given to advanced analytical techniques in biotechnology during the production of recombinant glycoproteins and other therapeutics. Hailed as "indispensable" in its first edition, Glycoanalysis Protocols, 2nd ed., continues with vital, time-tested techniques addressing the needs of both biomedical researchers and protein macromolecular structural chemists. It will well serve all those starting work on the analysis of glycoproteins, as well as more experienced investigators seeking to augment their expertise.

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Characterization of Protein Glycosylation, E. F. Hounsell. Analytical Methods for the Study of O-GlcNAc Glycoproteins and Glycopeptides, K. D. Greis and G. W. Hart. Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography, K. Yamamoto, T. Tsuji, and T. Osawa. Exoglycosidase Sequencing of N-Linked Glycans by the Reagent Analysis Method (RAAM), S. Prime and T. Merry. HPAE-PAD Analysis of Monosaccharides Released by Exoglycosidase Digestion Using the Carbopac MA1 Column, M. Weitzhandler, J. Rohrer, J. R. Thayer, and N. Avdalovic. HPLC and HPAEC of Oligosaccharides and Glycopeptides, M. J. Davies and E. F. Hounsell. Analysis of N- and O-Glycans by Pyridylamination, S. Natsuka and S. Hase. Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins, J. C. Klock and C. M. Starr. Analysis of Glycosaminoglycans and Proteoglycans, C. C. Rider. The Use of Cell and Organ Culture for the Study of Secreted Mucins, A. P. Corfield, A. Aslam, S. Wood, B. Singh, and C. Paraskeva. Purification of Gastrointestinal Mucins and Analysis of Their O-Linked Oligosaccharides, B. J. Campbell and J. M. Rhodes. TLC in Structure and Recognition Studies of Glycosphingolipids, J. Müthing. Strategies for Preliminary Characterization of Novel Amphoteric Glycosphingolipids, R. D. Dennis, G. Lochnit, and R. Geyer. Analysis of the Carbohydrate and Lipid Components of Glycosylphosphatidylinositol Structures, A. Treumann, M. Lucia, S. Güther, P. Schneider, and M. A. J. Ferguson. Conformational Analysis of Biantennary Glycopeptides with a Resonance Energy Transfer Technique, K. B. Lee, P. Wu, L. Brand, and Y. C. Lee. Index.