The objective of this volume is to provide readers with a current view of all aspects of the 'pipeline' that takes protein targets to structures and how these have been optimised. This volume includes chapters describing, in-depth, the individual steps in the Structural Genomics pipeline, as well as less detailed overviews of individual Structural Genomics initiatives. It is the first book of protocols to cover techniques in a new and emerging field.

Hersteller:
Humana Press in Springer Science + Business Media
juergen.hartmann@springer.com
Heidelberger Platz 3
DE 14197 Berlin

Section 1: Protein target selection, bioinformatic approaches and data management Target selection for structural genomics: An Overview. Russell L. Marsden and Christine A. Orengo A General target selection method for crystallographic proteomics. Gautier Robin, Nathan P. Cowieson, Gregor Guncar, Jade Forwood, Pawel Listwan, David A. Hume, Bostjan Kobe, Jennifer L. Martin, Thomas Huber Target Selection: Triage in the Structural Genomics Battlefield. James Raftery. Data Management in Structural Genomics: An Overview. S. Haquin, E. Oeuillet, A. Pajon, M. Harris, A.T. Jones, H. van Tilbeurgh, J.L. Markley, Z. Zolnai, and A. Poupon Data deposition and annotation at the Worldwide Protein Data Bank. Shuchismita Dutta, Kyle Burkhardt, Ganesh J. Swaminathan, Takashi Kosada, Kim Henrick, Haruki Nakamura, Helen M. Berman Prediction of protein disorder. Zsuzsanna Dosztanyi and Peter Tompa Protein domain prediction. Helgi Ingolfsson and Golan Yona Protein Structure Modeling With MODELLER. Narayanan Eswar, David Eramian, Ben Webb, Min-Yi Shen and Andrej Sali Section 2: Protein production High throughput cloning with restriction enzymes. Volker Sievert, Asgar Ergin and Konrad Büssow Automated recombinant protein expression screening in Escherichia coli. Didier Busso, Matthieu Stierle, Jean-Claude Thierry and Dino Moras From no expression to high-level soluble expression in Escherichia coli by screening a library of the target protein with randomized N-terminus. Kyoung Hoon Kim, Jin Kuk Yang, Geoffrey S. Waldo, Thomas C. Terwilliger, and Se Won Suh Application of High-Throughput Methodologies to the Expression of Recombinant Proteins in E. coli. Yoav Peleg and Tamar Unger A high-throughput platform for eukaryotic genes. Yunjia Chen, Shihong Qiu, Chi-Hao Luan and Ming Luo High-Throughput production of recombinant human proteins for crystallography. Opher Gileadi, Nicola A. Burgess-Brown, Steve M. Colebrook, Georgina Berridge, Pavel Savitsky, Carol E.A. Smee, Peter Loppnau, Catrine Johansson, Eidarus Salah, Nadia H. Pantic Assembly of protein complexes by co-expression in prokaryotic and eukaryotic hosts. Anastassis Perrakis and Christophe Romier Cell-free protein synthesis for analysis by NMR spectroscopy. Margit A. Apponyi, Kiyoshi Ozawa, Nicholas E. Dixon and Gottfried Otting A medium or high-throughput protein refolding assay. Nathan P. Cowieson, Beth Wensley, Gautier Robin, Gregor Guncar, Jade Forwood, David A. Hume, Bostjan Kobe and Jennifer L. Martin Structural Proteomics of Membrane Proteins: A Survey of Published Techniques and Design of a Rational High-Throughput Strategy. Melissa Swope Willis and Christopher M. Koth Section 3: Biophysical and functional chatacterization of proteins Methods for protein characterization by mass spectrometry, thermal shift (ThermoFluor) assay, and multi-angle or static light scattering. Joanne E. Nettleship, James Brown, Matthew R. Groves and Arie Geerlof High-Throughput Methods for Analyzing Transition Metals in Proteins on a Microgram Scale. Anelia Atanassova, Martin Högbom and Deborah B. Zamble High Throughput Screening of Purified Proteins for Enzymatic Activity. Michael Proudfoot, Ekaterina Kuznetsova, Stephen A. Sanders, Claudio F. Gonzalez, Greg Brown, Aled M. Edwards, Cheryl H. Arrowsmith, Alexander F. Yakunin Section 4: Structural characterization of proteins Strategies for Improving Crystallization Success. Rebecca Page Protein crystallization in restricted geometry: Advancing old ideas for modern times in structural proteomics. Joseph D. Ng, Raymond C. Stevens and Pete ...