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Parallel Single Cell Electrophoresis in a Microchip Platform

Erschienen am 25.08.2009
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Bibliografische Daten
ISBN/EAN: 9783838304489
Sprache: Englisch
Umfang: 140 S.
Format (T/L/B): 0.9 x 22 x 15 cm
Einband: kartoniertes Buch

Beschreibung

Single cell analysis has typically been difficult and challenging to perform in such a way that meaningful statistical information can be extracted. This thesis documents the development of a microchip or lab-on-a-chip platform to perform single cell electrophoresis in parallel separation channels. It describes the use of optical tweezers to select a cell and transport it to a desired separation channel. It also describes the use of electromechanical shearing to lyse a cell. These two techniques are combined with laser-micromachined separation channels to perform simple electrohoresis on the fluorescently labeled cellular contents.

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Autorenportrait

Nigel Munce is currently a research scientist and medical studentat the University of Toronto in Canada. He is interested in both medical devices andthe development of new medical imaging techniques.

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